Re: [Histonet] custom antibodies



We had very good service from ProSci. Here is their web site address.

If you are going invest in peptides I recommend immunizing each rabbit 
(or mouse) with at least three different peptides from your protein.  
This will increase your chances of a good antibody and lower the cost.  
You would need to analyze the protein sequence on a hydrophobicity plot 
to find good candidates for antigenicity.  I am sure ProSci (for a 
fee), can do this analysis as well.
Good luck,

On Mar 11, 2004, at 11:38 AM, Harvey, Jennifer wrote:

> Hi,
> We are interested in getting a custom antibody made, including the 
> peptide.
> Has anyone in histoland had this done? Do you recommend a company you 
> had
> luck with? I saw that Zymed makes both the peptide and the antibodies. 
> Has
> anyone ever used them?
> Thanks for you help.
> Jennifer Harvey
> Center for Cellular and Molecular Cardiology
> University of Rochester Medical Center
> 601 Elmwood Avenue, Box 679-CCMC
> Rochester, NY  14642
> Telephone: (585) 273-5161
> _______________________________________________
> Histonet mailing list
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