I will add that I know our insurance carriers will not reimburse for the
modifier 22 with IHC. I am sure that this is variable across the country,
and can only relate our experience.

Patti Loykasek
PhenoPath Laboratories
Seattle, WA>




 We had a very enlightening call from the Erbitux drug rep last week, and
> from her statement, the drug will not even be offered to any patient without
> documentation that the DAKO kit was used.  I have had to supply that
> information to our research people who enter patients on the protocols
> Possibly it might be due to the "newness" of this kit, but probably, from
> Patti's numbers, reimbursement will be a big issue.  We were also advised to
> use charge code 88342-22 to increase our reimbursement.
> 
> Jacquie Poteete MT(ASCP)QIHC, Lead Technologist, IHC Laboratory
> Saint Francis Hospital, Tulsa, OK
> japoteete@saintfrancis.com
> 
>> -----Original Message-----
>> From:    Sebree Linda A. [SMTP:la.sebree@hosp.wisc.edu]
>> Sent:    Friday, March 05, 2004 8:14 AM
>> To:    Histonet (E-mail)
>> Subject:    [Histonet] FW: [IHCRG] Very important
>> question!!!!!!!!!!!!!!!!
>> 
>> I thought these communiqués should be shared with the Histonet list
>> server.
>> 
>> Linda A. Sebree
>> University of Wisconsin Hospital & Clinics
>> IHC/ISH Clinical & Research Laboratory
>> DM223-VA
>> 600 Highland Ave.
>> Madison, WI 53792
>> (608)265-6596
>> FAX:  (608)262-7174
>> 
>> 
>> 
>> -----Original Message-----
>> From: lualhati harkins [mailto:leharkins@yahoo.com]
>> Sent: Thursday, March 04, 2004 6:23 PM
>> To: Patsy Ruegg; Rcartun@harthosp.org; deb.vaneyck@phci.org;
>> ihcrg@yahoogroups.com
>> Cc: brian.kueck@phci.org; david.ferguson@phci.org;
>> katherine.bayliss@phci.org
>> Subject: RE: [IHCRG] Very important question!!!!!!!!!!!!!!!!
>> 
>> 
>> HI Patsy,
>>  In addition to EGFr issue, i use Egfr antibody from Zymed and our
>> pathologist like it. I also tried polyclonal  from Bio Care and it is very
>> good too. Currently, we do not have plans to evaluate and EGFR kit, we are
>> confident with our developed protocol for the EGFr antibody we are
>> currently using. We did pretty good with The CAP Survey samples using
>> EGFR. We placed confidently in good percent of agreement with results from
>> other labs.
>> Thank you,
>> Loui
>> 
>> Patsy Ruegg  wrote:
>> Yes the rab. monoclonal egfr I am using is a sample from Lab Vision.  Have
>> 
>> you used it?  Any pointers?
>> Patsy
>> 
>> 
>> 
>> Patsy Ruegg, HT(ASCP)QIHC
>> IHCtech, LLC
>> Fitzsimmons BioScience Park
>> 12635 Montview Blvd. Suite 216
>> Aurora, CO 80010
>> P-720-859-4060
>> F-720-859-4110
>> HP-303-644-4538
>> 
>> hm email pruegg@msn.com
>> wk email pruegg@colobio.com
>> 
>> This email is confidential and intended solely for the use of the
>> Person(s) 
>> ('the intended recipient') to whom it was addressed. Any views or opinions
>> 
>> presented are solely those of the author. It may contain information that
>> is 
>> privileged & confidential within the meaning of applicable law.
>> Accordingly 
>> any dissemination, distribution, copying, or other use of this message, or
>> 
>> any of its contents, by any person other than the intended recipient may
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>> If 
>> you are NOT the intended recipient please contact the sender and dispose
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>> 
>> 
>> 
>> 
>> 
>> ----Original Message Follows----
>> From: "Richard Cartun" 
>> To: ,, 
>> CC: 
>> ,,> rg>
>> Subject: RE: [IHCRG] Very important question!!!!!!!!!!!!!!!!
>> Date: Thu, 04 Mar 2004 14:55:46 -0500
>> 
>> Yes, and it's very expensive!  We evaluated their EGFr kit a while ago
>> along with two other mAbs (BioGenex and Zymed).  We actually prefer the
>> Zymed antibody.  Patsy, is the rabbit mAb that you are using from Lab
>> Vision?
>> 
>> Rich Cartun
>> 
>>>>> "Patsy Ruegg"  03/04/04 02:11PM >>>
>> Deb,
>> This is a very important issue and I hope that we all get fed back on
>> this.
>> I am using the rabbit monoclonal egfr antibody on  some research
>> samples,
>> but not the kit.  I have very little data yet to report.  Is DAKO
>> selling
>> the EGFR kit?
>> Patsy
>> 
>> 
>> 
>> Patsy Ruegg, HT(ASCP)QIHC
>> IHCtech, LLC
>> Fitzsimmons BioScience Park
>> 12635 Montview Blvd. Suite 216
>> Aurora, CO 80010
>> P-720-859-4060
>> F-720-859-4110
>> HP-303-644-4538
>> 
>> hm email pruegg@msn.com
>> wk email pruegg@colobio.com
>> 
>> This email is confidential and intended solely for the use of the
>> Person(s)
>> ('the intended recipient') to whom it was addressed. Any views or
>> opinions
>> presented are solely those of the author. It may contain information
>> that is
>> privileged & confidential within the meaning of applicable law.
>> Accordingly
>> any dissemination, distribution, copying, or other use of this message,
>> or
>> any of its contents, by any person other than the intended recipient
>> may
>> constitute a breach of civil or criminal law and is strictly
>> prohibited. If
>> you are NOT the intended recipient please contact the sender and
>> dispose of
>> this e-mail as soon as possible.
>> 
>> 
>> 
>> 
>> 
>> ----Original Message Follows----
>> From: "Van Eyck, Deb" 
>> To: "'ihcrg'" 
>> CC: "Kueck, Brian D." ,   "Bayliss, Katherine"
>> ,   "Ferguson, David J."
>> 
>> Subject: [IHCRG] Very important question!!!!!!!!!!!!!!!!
>> Date: Thu, 4 Mar 2004 10:26:38 -0600
>> 
>> Hi and I would like feedback from clinical folks out there--------have
>> you
>> started running your EGFR kits on patients yet????  How is the
>> staining????
>> I ran a number of random cases and seem to be getting darker staining
>> and
>> more cytoplasmic reaction than suggested.  My negs are great and
>> clean-kit
>> exactly followed.  My cervix is much darker than in picture in old
>> guide and
>> the diffuse cytoplasmic staining predominated most cases making the
>> membrane
>> staining hard to evaluate.  What is your experience?? Most of my cases
>> are 6
>> months or less tissue cases.
>> 
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>> ---------------------------------
>> Yahoo! Groups Links
>> 
>>    To visit your group on the web, go to:
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>> 
>> ****************************************************************
>> Please note: Protocol and technical information provided by the
>> undersigned are developed on site with conditions limited to fixation of
>> tissues,reagents,control tissues and execution of protocol which is
>> characteristic only of our laboratory and therefore we do not bear neither
>> responsibility nor liability for results outside of our laboratory. It is
>> our courteous gesture to share
>> information that we deemed might be useful to laboratorians and
>> technologists.
>> ******************************************************************
>> Loui Harkins , M.S. QIHC
>> Biologist, Immunohistochemistry/Path.& lab. Medicine
>> DEpt. Of Veterans Affairs,700 South, 19th Street
>> Birmingham, Alabama 35233
>> (Research Consultant) Dept .of Surgery, Neurosurgery Division
>> University of Alabama in Birmingham
>> 205-933-8101 ext.6719; fax 205-558-4817 or 558-7034
>> 
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