Re: [Histonet] Brucella staining
Phil and all,
The Putt Modification of the Zeihl-Neelsen Stain is reputed to stain bovine
The method is a cold ZN utilising New Fuchsin at room temperature and then
treating with Saturated Aqueous Lithium Carbonate.
The method is in "Animal Tissue Techniques" by Gretchen L. Humason.
At 10:13 AM 03/11/04 -0600, Philip Oshel wrote:
>I've got a project coming in to look at bacteria in the SEM. No problem, I
>do that routinely. But, it seems to me that this group would be better off
>sectioning their samples and staining the bacteria.
>Except they claim the bugs won't stain.
>This is for Brucella melitensis in mouse tails.
>Anyone have a good stain for these bacteria? The stain would be done on
>Calcified or decalified better? I don't think that matters to the study,
>but it might to the staining.
>Supervisor, BBPIC microscopy facility
>Department of Animal Sciences
>University of Wisconsin
>1675 Observatory Drive
>Madison, WI 53706 - 1284
>voice: (608) 263-4162
>fax: (608) 262-5157 (dept. fax)
>Histonet mailing list
Mr.Laurie Reilly Ph 07 4781 4468
Physiology & Pharmacology Fax 07 4779 1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
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