I'm with Jackie on the freeze/thaw thing. I worked in antibody manufacturing 
for several years, and while it was common to store antibodies frozen 
(generally -20 C), the critical thing we tried to avoid was multiple 
freeze/thaw cycles. We started to see fracturing of the antibodies after 
about 3-5 freeze/thaw cycles as well. So, I'd certainly recommend 
aliquotting, *especially* if you've got a small amount of customized Ab 
(monoclonal, custom conjugate, etc.) that you're using.

--Aloha to all,
Jack England

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