RE:[Histonet] Processing "plastics" / resins
Heres what we do:
After fixation place into
70% a few hrs
95% alc 10 min-1 hr
Absolute alc 10 min-1hr
Glycolmethacrylate (GMA) 1st change 30 mins-4 hrs
GMA 2 30 mins-overnight
GMA 3 30 mins-24 hrs
GMA 4 30 mins-24 hrs
Prepare new monomer 88ml 2-hydroxyethylmethacrylate
12ml 2 butoxyethanol
0.45g Benzoyl peroxide
Then add promoter to monomer as 1:50.
8 ml polyethylene glycol 400
1 ml NN Dimethylaniline
There is no clearing necessary. You can dip straight into your dye with
out hydrating the slides.
Hope that helps
Good morning everyone !
Excuse my ignorance on this issue,but I have never processed or stained
tissues with resins such as Glycolmethacrylate or Methylmethacrylate. Do
you normally run the tissues through a clearing agent for processing and
staining? I didn't think you needed to except for the end of the staining
process. If you do use a clearant in any step of the process, can you use a
Xylene substitute like Shandon Xylene substitute ?
Thanks in advance for your replies !
Histology Service Unit
University of Otago
(03) 479 7152
Histonet mailing list
<< Previous Message | Next Message >>