[Histonet] TUNEL (PROMEGA)
I'm doing TUNEL staining on mouse brains (formaldehyde fixed, paraffin
section) right now.
I have problem with non-specific binding of the cells; ie almost all cells
stained positive. I used the protocol given by Promega with some
modifications; I performed microwave antigen retrieval and block the tissues
with 20% NGS/1%BSA in PBS before adding TdT reaction mix.
Can anyone help me to solve the non-specific bindings I keep getting?
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