[Histonet] Quality of tissues: freeze - thaw - freeze

From:Daniel Eberhard

Dear Listers,
some month ago I prepared some mouse tissues for cryo-sectioning without 
fixing the tissues before.
Now I have found out that usual fixing of sections is not efficient for 
our purposes, and
whole mount fixation (e.g. with pFA) would have been much better.
Thus my question:
Have some of you ever thawed unfixed tissue blocks,
fixed the tissues and re-frozen them? Im afraid the
integrity of the tissue might be spoiled and of course:
some of the tissues are valuable and unique.
Any hints are welcome
thanks
Daniel.

-- 
                           (-)-(-)
---------------------------  \"/ ---
Dr. Daniel Eberhard          =V=

Developmental Biology 
& Molecular Pathology 

Graduate Programe on Pattern Formation

University of Bielefeld
D 33501 Bielefeld/Germany

FAX: xx49(0)521-106-5654   (-)-(-)
---------------------------  \"/ ---
                             =V=   




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