[Histonet] MicroFil

From:"Sven Terclavers"

Dear listers,

I'm looking for a solution to perfuse bloodvessels which hardens after
cooling down or after a certain timepoint of being liquid to fill not only
larger bloodvessels but also cappillars.  On the internet I found MicroFil,
now I was wondering if some of you already tried this.  I would like to know
the following:

-What is the smallest cappillar that will be filled?
-Can sections be made without the MicroFil falling out of the cappillars?
-Can this MicroFil solution be mixed with e.g. FITC to  perform
epi-fluorescence/CLS-microscopy?

Thanks in advance,

Sven Terclavers


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