[Histonet] FW: flow citometry protocol

From:"Serrano del Pozo, Erika"


> Hi histotechs,
> 
> I'm working with injured mice muscle, so it┤s full of inflammatory cells
> at 2-5 days after injury. I┤d like to know if anyone of you knows how to
> separate these inflammatory cells from a whole muscle to perform a flow
> citometry assay or where can I find a protocol to do it. Since now I've
> been doing IHC in frozen tissue because I only needed to check there were
> macrophages and T-lymphocytes, but now I need to count them and I don't
> know how to obtain "clean" cells to perform the experiment, I couldn┤t
> find anything on the internet.
> 
> Thanks in advance,
> 
> 
> 	           Erika Serrano
> 
>   Centre de Regulaciˇ Gen˛mica
> Differenciation and Cancer Programme
> 	              Barcelona 
> 
> 

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