I thought these communiqués should be shared with the Histonet list server.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: lualhati harkins [mailto:leharkins@yahoo.com]
Sent: Thursday, March 04, 2004 6:23 PM
To: Patsy Ruegg; Rcartun@harthosp.org; deb.vaneyck@phci.org;
ihcrg@yahoogroups.com
Cc: brian.kueck@phci.org; david.ferguson@phci.org;
katherine.bayliss@phci.org
Subject: RE: [IHCRG] Very important question!!!!!!!!!!!!!!!!


HI Patsy,
 In addition to EGFr issue, i use Egfr antibody from Zymed and our pathologist like it. I also tried polyclonal  from Bio Care and it is very good too. Currently, we do not have plans to evaluate and EGFR kit, we are confident with our developed protocol for the EGFr antibody we are currently using. We did pretty good with The CAP Survey samples using EGFR. We placed confidently in good percent of agreement with results from other labs.
Thank you,
Loui

Patsy Ruegg  wrote:
Yes the rab. monoclonal egfr I am using is a sample from Lab Vision.  Have 
you used it?  Any pointers?
Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
HP-303-644-4538

hm email pruegg@msn.com
wk email pruegg@colobio.com

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----Original Message Follows----
From: "Richard Cartun" 
To: ,, 
CC: 
,,
Subject: RE: [IHCRG] Very important question!!!!!!!!!!!!!!!!
Date: Thu, 04 Mar 2004 14:55:46 -0500

Yes, and it's very expensive!  We evaluated their EGFr kit a while ago
along with two other mAbs (BioGenex and Zymed).  We actually prefer the
Zymed antibody.  Patsy, is the rabbit mAb that you are using from Lab
Vision?

Rich Cartun

>>> "Patsy Ruegg"  03/04/04 02:11PM >>>
Deb,
This is a very important issue and I hope that we all get fed back on
this.
I am using the rabbit monoclonal egfr antibody on  some research
samples,
but not the kit.  I have very little data yet to report.  Is DAKO
selling
the EGFR kit?
Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
HP-303-644-4538

hm email pruegg@msn.com
wk email pruegg@colobio.com

This email is confidential and intended solely for the use of the
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privileged & confidential within the meaning of applicable law.
Accordingly
any dissemination, distribution, copying, or other use of this message,
or
any of its contents, by any person other than the intended recipient
may
constitute a breach of civil or criminal law and is strictly
prohibited. If
you are NOT the intended recipient please contact the sender and
dispose of
this e-mail as soon as possible.





----Original Message Follows----
From: "Van Eyck, Deb" 
To: "'ihcrg'" 
CC: "Kueck, Brian D." ,   "Bayliss, Katherine"
,   "Ferguson, David J."

Subject: [IHCRG] Very important question!!!!!!!!!!!!!!!!
Date: Thu, 4 Mar 2004 10:26:38 -0600

Hi and I would like feedback from clinical folks out there--------have
you
started running your EGFR kits on patients yet????  How is the
staining????
I ran a number of random cases and seem to be getting darker staining
and
more cytoplasmic reaction than suggested.  My negs are great and
clean-kit
exactly followed.  My cervix is much darker than in picture in old
guide and
the diffuse cytoplasmic staining predominated most cases making the
membrane
staining hard to evaluate.  What is your experience?? Most of my cases
are 6
months or less tissue cases.


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****************************************************************
Please note: Protocol and technical information provided by the undersigned are developed on site with conditions limited to fixation of tissues,reagents,control tissues and execution of protocol which is characteristic only of our laboratory and therefore we do not bear neither responsibility nor liability for results outside of our laboratory. It is our courteous gesture to share
information that we deemed might be useful to laboratorians and technologists.
******************************************************************
Loui Harkins , M.S. QIHC
Biologist, Immunohistochemistry/Path.& lab. Medicine
DEpt. Of Veterans Affairs,700 South, 19th Street
Birmingham, Alabama 35233
(Research Consultant) Dept .of Surgery, Neurosurgery Division
University of Alabama in Birmingham
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