Please pardon my ignorance, but what kind of frozen tissues are you 
sectioning on the sliding microtome, what brand & type sliding microtome 
are you using, and how are you keeping your samples frozen for sectioning 
on it? Thanks, Atoska


>X-Mailer: Novell GroupWise Internet Agent 6.5.1
>Date: Mon, 08 Mar 2004 13:36:00 -0500
>From: "Mollie Lange" 
>To: 
>X-Scan-Signature: 6e25c54ecfa6545cca04a3e1c9f5b689
>X-Content-Filtered-By: Mailman/MimeDel 2.1.3
>Cc:
>X-BeenThere: histonet@lists.utsouthwestern.edu
>X-Mailman-Version: 2.1.3
>List-Id: For the exchange of information pertaining to histotechnology and
>         related fields 
>List-Unsubscribe: 
>,
> 
>
>List-Archive: 
>List-Post: 
>List-Help: 
>List-Subscribe: ,
>         
>Sender: histonet-bounces@lists.utsouthwestern.edu
>X-Scan-Signature: 05628b3a735271e1629bc35bb8abda07
>X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu
>Subject: [Histonet] re: cryostat sectioning
>X-Spam-Checker-Version: SpamAssassin 2.63 (2004-01-11) on swlx162.swmed.edu
>X-Spam-Level:
>X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.63
>X-SA-Exim-Version: 3.1 (built Tue Oct 14 16:21:02 CDT 2003)
>X-SA-Exim-Scanned: Yes
>
>Hi Kathleen,
>
>I feel your pain.  I've been cutting cryostat sections for almost 30
>years, and sometimes it can be completely maddening for no discernible
>reason.
>
>It could possibly be static as one Histonetter suggested.  A Japanese
>fellow in our lab showed us that spraying Cytocool on the knife and roll
>plate really works.  It's the ONLY thing that consistently works.
>
>Are your tissue blocks cryoprotected in sucrose?  Have you tried a
>freshly made solution of your cryoprotectant in case the original was
>not made proplerly?
>
>We are a research lab, and we always cut our fixed frozen sections for
>immuno on a sliding microtome.  We find this method much easier than the
>cryostat for fixed frozen. Do you have a sliding microtome available and
>have you tried it?
>
>Good luck,
>Mollie Lange
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet