staining too weak for ISH
Message for Sanjay about weak counterstaining of is situ hybridization slides
Hi, I do a H&E counterstain on my radio-labelled in situ slides. But I
found the hematoxylin was too weak so I purchased a Gill triple strength
and an intensified eosin. I stain for 1 minute each. The stain is
beautiful. In comparison, if I use this same hematoxylin for an IHC
counterstain, I can do one quick dip and that is more than enough! Staining
5-10 minutes in Mayer's Hematoxylin did not stain intensely enough for my
ISH. So, I think you need a more concentrated stain. Terri Thinnes The
Scripps Research Institute
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