b-gal staining in old bones
I have the following problem. I have an old mouse expressing LacZ (a
transgene) and I would like to see where the LacZ is expressed. Soft
organs (heart, lungs) I can do wholemount and then slice it up. The
problem is with bone. Cannot do wholemounts, too high mineral content. I
cannot demineralize because then the divalent cations would be gone and
my b-gal would destabilize (or would it?). I cannot make frozen sections
and if I do paraffin sections then b-gal is dead.
Any help, experience, pointers will be appreciated. BTW, I googled
around, checked the library and talked to some people. So far I have no
solution to my problem.
Przemko Tylzanowski Ph.D.
LSD & Joint
O & N
University of Leuven
fax : (32-16)34-62-00
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