b-gal staining in old bones


I have the following problem. I have an old mouse expressing LacZ (a 
transgene) and I would like to see where the LacZ is expressed. Soft 
organs (heart, lungs) I can do wholemount and then slice it up. The 
problem is with bone. Cannot do wholemounts, too high mineral content. I 
cannot demineralize because then the divalent cations would be gone and 
my b-gal would destabilize (or would it?). I cannot make frozen sections 
and if I do paraffin sections then b-gal is dead.
Any help, experience, pointers will be appreciated. BTW, I googled 
around, checked the library and talked to some people. So far I have no 
solution to my problem.


Przemko Tylzanowski Ph.D.
LSD & Joint
O & N
University of Leuven
Herestraat 49
3000 Leuven

phone: (32-16)34-61-96
fax  : (32-16)34-62-00

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