This is the protocol I have.  I have also seen it referred 
to as picrisirius.

SIRIUS RED (for Collagen)
Fix specimen in 10% Buffered Formalin, embed in paraffin, 
section at 5 microns.

1.	Hemo-de	5 min.
2.	Hemo-de	5 min.
3.	Hemo-de	5 min.
4.	100% EtOH	3 min.
5.	100% EtOH	3 min.
6.	95% EtOH	3 min.
7.	95% EtOH	3 min.
8.	80% EtOH	3 min.
9.	Tap water wash	3 min.
5.	0.1% Sirius Red	30 min.
15.	80% EtOH	1 min.
16.	95% EtOH	1 min.
17.	95% EtOH	1 min.
18.	100% EtOH	10 min.
19.	100% EtOH	10 min.
20.	Hemo-de	3 min.
21.	Hemo-de	3 min.
22.	Hemo-de	3 min.
23.	Mount with mounting media


Results:
	light microscopy:	collagenous fibers	- red
	other tissue elements	- bright yellow

	polarized microscopy: 	collagen fibers	- orange/red bands
	against black background


0.1% Sirius Red Solution
Sirius Red dye	0.1 gm.
Picric Acid saturated 	100 ml.

Store in refrigerator.  Stable for 4-6 months.

Reference:  Greenberg, Stephen, Ph.D., Laboratory Medicine, 
Vol. 17, No. 8, August, 1986.

Karen Bowden
UCSD
Ortho Dept.


Liz Chlipala wrote:
> Has anyone heard of a stain that contains picric acid and sirius red.  I'm
> assuming that its a stain for collagen.  The spelling I got from a client
> was picrisirius.
> 
> Thanks in advance
> 
>