Re: loss of antigenicity


I perform Ki67 and CD31 extensively on paraffin sections.   I have noted a marked decrease in antigenicity in sections stored at RT cut just one week prior to staining.  I make it a point to perform Ki67 within 3 days of sectioning.   I HAVE NOT noticed a decrease in CD31 sections (stored at room temp) cut more than 2 months prior to staining.  If someone else has noticed loss of antigenicity, I'd appreciate the data as well.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
Fax 847.938.3266


"Connolly, Brett M" <>

03/20/2003 02:01 PM

        To:        "'HISTONET' (E-mail)" <>
        Subject:        loss of antigenicity

I am looking for references for decreases of antigenicity in paraffin
sections stored over temp, 4C or both. Of particular interest
are Ki-67 and CD31. I only found one in a pubmed search. Can you folks could
help me out? Internal observations would also be useful.

Brett M. Connolly, Ph.D.
Merck & Co.,Inc.
MRL, Imaging
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075

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