on 3/27/03 5:28 AM, Michael A Kuluz at kuluz001@mc.duke.edu wrote:

> 
> 
> 
> 
> Does anyone have a protocol for using rat anti-mouse cd11b to stain
> paraffin embedded neonatal mouse brain?  I have tried once and before we
> embark on another failure, I thought I'd ask here. Thanks in advance.
> 
> Mike Kuluz
> Duke University
> kuluz001@mc.duke.edu
> 
> 
Mike

If you are using the antibody from pharmingen cat # 550282 I believe it only
works in frozen sections.  We run that antibody here with good results on
paraformaldehyde fixed frozen sections.


-- 

Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory
P.O. Box 18592
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lizchlipala@premierhistology.com
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