Re: Protocol for staining mouse brain for microglia...
on 3/27/03 5:28 AM, Michael A Kuluz at firstname.lastname@example.org wrote:
> Does anyone have a protocol for using rat anti-mouse cd11b to stain
> paraffin embedded neonatal mouse brain? I have tried once and before we
> embark on another failure, I thought I'd ask here. Thanks in advance.
> Mike Kuluz
> Duke University
If you are using the antibody from pharmingen cat # 550282 I believe it only
works in frozen sections. We run that antibody here with good results on
paraformaldehyde fixed frozen sections.
Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
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