I was never able to find a difference. You could use whichever protocol is
more convenient for you, I think.

Lesley Weston.


on 28/03/2003 2:05 AM, Fernando Capela e Silva at fcs@uevora.pt wrote:

> What is the best in immnuohistochemestry:
> 
> - primary antibody incubation overnight at 4 degrees ?
> 
> - primary antibody incubation 30-60 min at room temperature ?
> 
> With compliments
> 
> * * * * * * * * * * * * * * * * * * * * * * * * *
> Fernando Capela e Silva
> Laborat#243#rio de Biologia da Conserva#231##227#o
> Departamento de Biologia
> Universidade de ...vora
> Apartado 94
> 7002-554 ...vora
> PORTUGAL
> 
> Phone: +351-266 760 800
> Fax: +351-266 711 231
> Email: fcs@uevora.pt
> 
> http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm
> 
> 
>