Re: Pease

From:Lesley Weston

Re: Pease

-----Original Message-----
From: Geoff McAuliffe []
Sent: 27 March 2003 21:59
To: J. A. Kiernan
Cc: Lorraine Gibbs;
Subject: Re: Pease

   A quick scan (not a thorough read) of the fixation chapter in Pease's book suggests it might be a buffered osmium tetroxide fixative. You could look up Pease and Baker, 1950, Am. J. Anat. 87:349.

J. A. Kiernan wrote:
Lorraine Gibbs wrote:
Does anyone out there know of a Pease fixative?

Yes: honey.

I eat my peas with honey.
I've done it all my life.
It makes the peas taste funny
But it keeps them on the knife.

Otherwise it might be something in "Histological
Techniques for Electron Microscopy" by D.C.Pease,
Academic Press, New York, 1964. I don't have a
copy, but do have one of Hayat's EM books (1981),
which doesn't have an eponymous fixative Pease
fixative in the index.

It is. I finally found my copy, and he describes it in chapter 3.9, giving the reference as :

Pease D.C. (1962). Anat. Record 142 342.

It's a formaldehyde fixative using the same buffer that Millonig used for OsO4 fixation, to reduce the disturbance caused by changing from one fixative to the next. Hope this helps.

Lesley Weston.

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