Re: Infarct and Trichrome Questions

From:Geoff McAuliffe

The time frame you describe is not long enough for new collagen to form and show up with a trichrome stain. I recall that there are stains to show infarcted myocardium, but I can't remember any specifics. I suggest a trip to the library. Look in textbooks of  Pathology, Histopathology or Cardiology. Try Thompson's "Selected Histochemical and Histopathological Techniques" or Lillie and Fullmer's text for starters.

K.C. Rosburg wrote:
I am new at performing histology slides and have been appointed to do this for my lab. My current project is looking at the apoptotic rate of murine heart sections. I need a way to test and locate the area of the infarct at a cellular level.  The following protocol that is used on the slides is a TUNEL assay kit.  A friend suggested that I try using Massons TriChrome Blue.  This would stain the infarct area blue from the collagen.  But I am cant figure out how long it would take before the collagen begins to form following the infarct.  I am looking at the hearts roughly 12-16 hours after the infarct was induced.  Any thoughts as to how I can locate the infarct????
Help would be greatly apreciated

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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