Re: Infarct and Trichrome Questions
The time frame you describe is not long enough for new collagen to form and
show up with a trichrome stain. I recall that there are stains to show infarcted
myocardium, but I can't remember any specifics. I suggest a trip to the library.
Look in textbooks of Pathology, Histopathology or Cardiology. Try Thompson's
"Selected Histochemical and Histopathological Techniques" or Lillie
and Fullmer's text for starters.
K.C. Rosburg wrote:
I am new at performing histology slides and have been appointed to do this for my lab. My current project is looking at the apoptotic rate of murine heart sections. I need a way to test and locate the area of the infarct at a cellular level. The following protocol that is used on the slides is a TUNEL assay kit. A friend suggested that I try using Massons TriChrome Blue. This would stain the infarct area blue from the collagen. But I am cant figure out how long it would take before the collagen begins to form following the infarct. I am looking at the hearts roughly 12-16 hours after the infarct was induced. Any thoughts as to how I can locate the infarct????
Help would be greatly apreciated
Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
<< Previous Message | Next Message >>