Re: Ferritin and prussion blue

From:"J. A. Kiernan"

Ferritin in fixed tissue should stain nicely with the 
Prussian blue method of Perls, used in the usual way. 
Exposure to acid before staining (decalcification, for 
example) would extract the iron. The acid mixed with 
the potassium ferrocyanide in Perls' reagent releases
iron(III) ions from ferritin and also ensures that
the Prussian blue product precipitates properly. 
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Sven Terclavers wrote:
> Dear all,
> Does anyone know whether it is possible that ferritin, or the iron-part, disappears after an acidic
> or alcoholic step (or another step) in the staining-protocol for prussion blue or due to the
> tissuepreperation-protocol for sectioning (paraffin)?
> We are trying to stain bloodvessels that are mechanically filled with ferritin-gel with prussion
> blue.  Before staining it is clear that the ferritin is in the vessels, but after sectioning and
> staining it looks like the ferritin is gone.  Normally the prussion blue should stain the ferritin?!
> And it would be strange that it 'falls out' of the vessels during sectioning, since bismuth can be
> used nicely...
> Thanks in advance,
> Sven Terclavers

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