Re: Background staining query

From:Vinnie Della Speranza

We routinely include eosin in our biopsy processors and have not had any problem with our  ihc stlides which are clean. I recommend considering other causes for the blush you refer to.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>> Gary Gill  03/28/03 08:48AM >>>
I've been asked to post this question: "Does staining tissue with eosin
during processing [i.e., paraffin embedding] cause a background or "Blush"
for IHC?  This is related to paraffin embedded tissue cut at 3 microns."

Any help will be appreciated.  Thanks.

Gary Gill

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