RE: loss of antigenicity

From:Young Kwun

Hi,
I know one such reference.
"Assessment of Problems in Diagnostic and Research IHC Associated with
Epitope Instability in Stored Paraffin Sections" by Lambert JCM et al. in
Applied Immunohistochemistry & molecular Morphology 8(4):316-321, 2000
Yes, they mentioned Ki-67 annd CD31 along with many other antibodies. They
reported some storage damage on both antibodies. At the moment we only keep
some antobodies such as ER, PR and mismatch repair gene proteins at freezer
section.
I hope this helps.

Cheers






Young Kwun
Senior Hospital Scientist
Dept. of Anatomical Pathology
Concord Hospital
Concord NSW 2139 Australia
Tel)61-2-9767-6075
Fax)61-2-9767-8427
kwuny@email.cs.nsw.gov.au

"the universe we observe has precisely the properties we should expect if
there is, at bottom, no design, no purpose, no evil and no good, nothing but
blind, pitiless indifference."
"To err is human, to forgive is even more human"

The information contained in this message is intended for the named
addressee only, and is confidential to the sender and intended recipient. If
you are not the named addressee please do not copy, distribute, take any
action reliant on, or disclose anything in this E-mail message to any other
person or organisation. If you have received this message in error please
delete the email and notify me immediately. Views expressed in this message
are those of the individual sender and are not necessarily the views of
Central Sydney Area Health Service.



> -----Original Message-----
> From: Connolly, Brett M [mailto:brett_connolly@merck.com]
> Sent: Friday, 21 March 2003 07:02
> To: 'HISTONET' (E-mail)
> Subject: loss of antigenicity
>
>
> H'netters,
> I am looking for references for decreases of antigenicity in paraffin
> sections stored over time...room temp, 4C or both. Of particular interest
> are Ki-67 and CD31. I only found one in a pubmed search. Can you
> folks could
> help me out? Internal observations would also be useful.
> Thanks,
> Brett
>
> Brett M. Connolly, Ph.D.
> Merck & Co.,Inc.
> MRL, Imaging
> WP26A-3000
> PO Box 4
> West Point, PA 19486
> PH 215-652-2501
> fax. 215-652-2075
> e-mail. brett_connolly@merck.com
>
>
> ------------------------------------------------------------------
> ------------
> Notice: This e-mail message, together with any attachments,
> contains information of Merck & Co., Inc. (Whitehouse Station,
> New Jersey, USA) that may be confidential, proprietary
> copyrighted and/or legally privileged, and is intended solely for
> the use of the individual or entity named on this message. If you
> are not the intended recipient, and have received this message in
> error, please immediately return this by e-mail and then delete it.
>
> ==================================================================
> ============
>
>
>




<< Previous Message | Next Message >>