Ah. Tim's comments are correct. I was thinking of a method to expose 
the internal structures of samples without sectioning.
Freeze-fracture in this sense is very different and is used for 
membranes, lipids, and the like. Today's Monday for me, and I wrote 
too soon.
Phil

Freeze-fracture is used for EM, but you have to have very specialized 
equipment to do it. If you don't have the equipment you can probably 
find a university in the Boston area that has it.

Tim Morken
Atlanta

-----Original Message-----
From: DLongWoodward@aol.com [mailto:DLongWoodward@aol.com]
Sent: Monday, March 17, 2003 9:17 PM
To: histonet@pathology.swmed.edu
Subject: freeze fracturing skin samples

Hi,
I've been asked to learn how to freeze fracture skin samples.  HELP!
Anybody out there know how to do this and can send me a protocol?
thanks a bunch,
Denise Long Woodward
Harvard Skin Disease Research Center
Brigham & Women's Hospital
Boston, MA


-- 
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)