RE: freeze fracturing skin samples

From:Philip Oshel

Ah. Tim's comments are correct. I was thinking of a method to expose 
the internal structures of samples without sectioning.
Freeze-fracture in this sense is very different and is used for 
membranes, lipids, and the like. Today's Monday for me, and I wrote 
too soon.

Freeze-fracture is used for EM, but you have to have very specialized 
equipment to do it. If you don't have the equipment you can probably 
find a university in the Boston area that has it.

Tim Morken

-----Original Message-----
From: []
Sent: Monday, March 17, 2003 9:17 PM
Subject: freeze fracturing skin samples

I've been asked to learn how to freeze fracture skin samples.  HELP!
Anybody out there know how to do this and can send me a protocol?
thanks a bunch,
Denise Long Woodward
Harvard Skin Disease Research Center
Brigham & Women's Hospital
Boston, MA

Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)

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