RE: Tyrosinase Protocol

From:"Breckenridge, Richard A."
We use clone T311 from NovoCastra. We use it at a dilution of 1:25. We also use HIER for 20 min. in a pH 6.0 citrate buffer at 99 degrees and let cool for 20 min. on the counter. We run it on the DAKO using Alk Phos. (we use the Signet USA kit with Fast Red) We currently do not have a pic of this stain on our website, however, we do have a lot of other pics and have added nearly all the spec sheets (in .pdf format) for our antibodies. see them at www2.utmb.edu/pps . Follow the "SPECIAL PROCEDURES" link on the left hand side.
 
Sincerely,
 

Richard A. Breckenridge HT(ASCP)
Technical Supervisor
UTMB Special Procedures Laboratory
Clinical Sciences Building Room 504
Galveston, TX  77555-0748
Phone 409-772-8051   Fax 409-772-8030

-----Original Message-----
From: edison narvaez [mailto:connhist@yahoo.com]
Sent: Wednesday, March 26, 2003 11:42 AM
To: histonet@pathology.swmed.edu
Subject: Tyrosinase Protocol

would anybody share their protocol for Tyrosinase, we have not been able to get this AB to work.

We use the Dako Autostainer, water bath method for HIER, and envision polymer from Dako.

Thank you....


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