The blocks may be too cold when you are sectioning them.  I chill blocks of CNS with the tissue facing up, avoiding direct contact with the ice.  Spinal cord seems more prone to "blow up" on the water bath, lowering the bath temperature is a good idea.  Ammonia water ( about 5%) is good, I also use fabric softener.  Hope this helps.
 
Fred

	-----Original Message-----
From: THERESA ROHR  
Sent: Monday, March 17, 2003 4:11 PM
To: 
Subject: Spinal Cord



	Dear Histonetters: 

	We are having a very difficult time cutting some spinal cord. Even after 
long soaking, it is chattering and "cracking". What is a good softener to 
use after processing to get a really good section?  I seem to remember using 
Ammonia in distilled Water.  Does that sound familiar? 
I would really appreciate any advice. 

	Thanks, 
Theresa Rohr, BA HT(ASCP) 
Section Head, Nyack Hospital NY 






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