RE: Pease's fixative
Thanks for all the replies! I can now tell someone how to dispose of an old vial of (no-longer-a-mystery) Pease's fix.
On Thu, 27 Mar 2003, Smith, Allen wrote:
> Page 52 of the second edition (Academic Press, New York, 1964) of Daniel C.
> Pease's HISTOLOGICAL TECHNIQUES FOR ELECTRON MICROSCOPY gives:
> 83 ml 2.26% sodium dihydrogen phosphate
> 17 ml 2.52% sodium hydroxide
> 11 ml methanol-free 40% aqueous formaldehyde
> Adjust pH to 7.2-7.4 if necessary.
> [Pease adds that 4 g paraformaldehyde heated to 60 C in 11 ml of very dilute
> NaOH can be used as the source of the formaldehyde.]
> Pease recommends fixing for 10 to 30 minutes and then postfixing in osmium
> Allen A. Smith, Ph.D.
> Barry University
> School of Graduate Medical Sciences
> Podiatric Medicine and Surgery
> Miami Shores, Florida 33161-6695
> -----Original Message-----
> From: J. A. Kiernan [mailto:email@example.com]
> Sent: Thursday, March 27, 2003 3:58 PM
> To: Lorraine Gibbs
> Cc: Histonet@pathology.swmed.edu
> Subject: Re: Pease
> > Lorraine Gibbs wrote:
> > Hello-
> > Does anyone out there know of a Pease fixative?
> Yes: honey.
> I eat my peas with honey.
> I've done it all my life.
> It makes the peas taste funny
> But it keeps them on the knife.
> Otherwise it might be something in "Histological
> Techniques for Electron Microscopy" by D.C.Pease,
> Academic Press, New York, 1964. I don't have a
> copy, but do have one of Hayat's EM books (1981),
> which doesn't have an eponymous fixative Pease
> fixative in the index.
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
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