RE: Pease

From:

"Smith, Allen"

Pease did introduce a fixative in a paper in the Anatomical Record, whose formula he repeated in his book, Histological Techniques for Electron Microscopy.

A.G.E. Pearse was an enzyme histochemist. He was not a great fan of fixation. None of the first three editions of his Histochemistry: Theoretical and Applied mention any fixative under his name. He did recommend Holt's fixative and Janigan's fixative for some purposes.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695

-----Original Message-----
From: Andrew Shand [mailto:Andrew.Shand@north-bristol.swest.nhs.uk]
Sent: Friday, March 28, 2003 3:41 AM
Cc: Histonet@pathology.swmed.edu
Subject: RE: Pease

Just so long as it's not a misprint that should be Pearse fixative. As in A G Everson Pearse.

Andy Shand
-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu]
Sent: 27 March 2003 21:59
To: J. A. Kiernan
Cc: Lorraine Gibbs; Histonet@pathology.swmed.edu
Subject: Re: Pease

A quick scan (not a thorough read) of the fixation chapter in Pease's book suggests it might be a buffered osmium tetroxide fixative. You could look up Pease and Baker, 1950, Am. J. Anat. 87:349.

J. A. Kiernan wrote:
Lorraine Gibbs wrote:
Hello-
Does anyone out there know of a Pease fixative?
    
Yes: honey.

I eat my peas with honey.
I've done it all my life.
It makes the peas taste funny
But it keeps them on the knife.

Otherwise it might be something in "Histological
Techniques for Electron Microscopy" by D.C.Pease,
Academic Press, New York, 1964. I don't have a
copy, but do have one of Hayat's EM books (1981),
which doesn't have an eponymous fixative Pease
fixative in the index.

  
Geoff
--
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Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
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