RE: Pease
| From: | "Monson, Frederick C." |
No, it
IS Daniel C. Pease (1964), Histological Techniques for Electron Microscopy
(2nd Ed.), Academic Press, NY, NY, and the fixative is called "Pease"s
Fixative". It goes like this on p. 52 of the above (in his section on
Buffered Aldehydes (pp. 51-56):
Stock Buffer Salt Solution:
Monosodium (monobasic) sodium
phosphate
2.26%
Stock alkali solution:
NaOH 2.52%
Fixative, 10% buffered Formalin
Monosodium phosphate
solution
83 ml
NaOH
solution 17
ml
Choice of:
Formalin (methanol free, 40%
formaldehyde)
11 ml
Paraformaldehyde (powder, pH adjustment
and
60oC necessary for
solution)
4 gm
Adjust pH to 7.2-7.4 as necessary
History: This is Pease's first mention of Paraformaldehyde (nothing
in his 1st Ed. in 1960) and he wrote this book during a period during which
Electron Microscopists were toiling away trying every Aldehydes in the Fisher
catalog in all possible concentrations to find one or a combination that did
really well for general, or specific, tissue fixation/preservation.
"Formalin", as we knew it in those days was always laced with 10+% of methanol
as a preservative and that is why the ambivalence to it in the above
recipe.
Hope this helps:
Fred
Monson
Frederick C. Monson, PhD
Center for Advanced Scientific
Imaging
Mail Drop: Geology
West Chester University
West Chester,
PA, 19383
http://darwin.wcupa.edu/casi/
Phone/FAX: 610-738-0437
Hello-
Does anyone out there know of a Pease
fixative?
Thanks!
Lorraine
UW
Physiology
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