RE: NADH vials

From:

Andrew Shand

We buy our NADH in bulk. Then aliquot into microtubes for storage in the fridge. The rest of our substrate is made up, aliquoted and stored in the freezer. When we want to do an NADH we heat that to 37c and add the NADH. Works every time - unlike the ATP's!

Andy Shand

-----Original Message-----
From: Melissa Jans [mailto:histomjans@yahoo.com]
Sent: 28 March 2003 12:57
To: histonet@pathology.swmed.edu
Subject: NADH vials

Hello,

We have been performing the NADH on muscle biopsies for quite some time now. We have always used the premeasured NADH vials from Sigma. They have now stopped selling these vials and only sell the reagent in powder(bulk) form. Now we are having trouble with this stain. Any suggestions...has anyone found an alternative source for these premeasured vials of NADH powder?

Thanks!

Melissa Jans

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