RE: Infarct and Trichrome Questions

From:"Georger, Mary"

A Movat's Pentachrome will work quite nicely, but at your timepoint I am not
sure you will see much yet. We show at that timepoint with GAP junction
antibodies, specifically Connexin 43.

Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642

> ----------
> From: 	Geoff McAuliffe
> Sent: 	Friday, March 21, 2003 9:59 AM
> To: 	K.C. Rosburg
> Cc:
> Subject: 	Re: Infarct and Trichrome Questions
> The time frame you describe is not long enough for new collagen to form
> and show up with a trichrome stain. I recall that there are stains to show
> infarcted myocardium, but I can't remember any specifics. I suggest a trip
> to the library. Look in textbooks of  Pathology, Histopathology or
> Cardiology. Try Thompson's "Selected Histochemical and Histopathological
> Techniques" or Lillie and Fullmer's text for starters. 
> K.C. Rosburg wrote:
> 	I am new at performing histology slides and have been appointed to
> do this for my lab. My current project is looking at the apoptotic rate of
> murine heart sections. I need a way to test and locate the area of the
> infarct at a cellular level.  The following protocol that is used on the
> slides is a TUNEL assay kit.  A friend suggested that I try using Massons
> TriChrome Blue.  This would stain the infarct area blue from the collagen.
> But I am cant figure out how long it would take before the collagen begins
> to form following the infarct.  I am looking at the hearts roughly 12-16
> hours after the infarct was induced.  Any thoughts as to how I can locate
> the infarct????
> 	Help would be greatly apreciated
> Geoff
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029 
> **********************************************

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