| From: | Tony Henwood |
Erica,
Is the problem with the stain or is it with the frozen section technique? Try freezing the tissue as rapidly as possible, fix the sections in methanol or formal-alcohol and then stain.
OR Upload some pics to Histonet so that we can have a look.
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
-----Original Message-----
From: Erica.Schaffer@aventis.com [mailto:Erica.Schaffer@aventis.com]
Sent: Saturday, 22 March 2003 7:58
To: histonet@pathology.swmed.edu
Subject: Hematoxylin & Eosin Staining on Frozen Brain
Hello,
I am trying to find an H&E protocol for staining frozen brain sections cut at
7 microns. I have tried to stain the samples using the same protocol I use
for other tissue types but I am not getting good results. If anyone has any
advice, I would really appreciate it.
Thank You,
Erica (Aventis Pharmaceuticals)
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