RE: Hematoxylin & Eosin Staining on Frozen Brain

From:Tony Henwood

RE: Hematoxylin & Eosin Staining on Frozen Brain


Is the problem with the stain or is it with the frozen section technique? Try freezing the tissue as rapidly as possible, fix the sections in methanol or formal-alcohol and then stain.

OR Upload some pics to Histonet so that we can have a look.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: []
Sent: Saturday, 22 March 2003 7:58
Subject: Hematoxylin & Eosin Staining on Frozen Brain

I am trying to find an H&E protocol for staining frozen brain sections cut at
7 microns. I have tried to stain the samples using the same protocol I use
for other tissue types but I am not getting good results. If anyone has any
advice, I would really appreciate it.

Thank You,
Erica (Aventis Pharmaceuticals)

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