I had the same problem also but it had to do with air pockets within the
heart that I embedding, even with the apex being cut off and vacuum infiltration.
 I read about doing perfusion but that is not a possibility in my case
Thanks


>-- Original Message --
>Date: Thu, 20 Mar 2003 21:24:18 -0500
>From: Cynthia Stone 
>Subject: Good Paraffin for TMA's
>To: HistoNet Server 
>
>
>Hi Histonetters,
>I have run into a problem with something to do with the embedding process.
>I have bubbles in the blocks after they come off the embedding center. 
I'm
>not sure if it's air in the line of the new embedding center (Sukura) or
>with the paraffin (Shandon).  Has anyone experienced any problems with the
>new embedding center?  Could that be what is making these bubbles?  Is it
>the paraffin, (I don't think so),  has anyone seen these bubbles form in
>paraffin as the block cool or even the next day? Lastly any suggestions
if
>I have to change paraffins as to which to try, needs to be hard to core
yet
>we have to be able to produce good sections at 4 microns for H&E's.  I don't
>process the tissue on site, so It's hard to trouble shoot but I have never
>seen these bubbles and I have been around for a long time.