RE: Background staining IHC cell blocks

From:"Morken, Tim"

ThermoShandon makes a kit called Cytoblock Kit, catNo 7401150, that is used
with their own embedding solution and specially made cassettes. We have used
it with good results

Tim Morken
CDC, Atlanta

-----Original Message-----
From: Julian-Boyd, Dawn [] 
Sent: Thursday, March 20, 2003 1:27 PM
To: ''
Subject: Background staining IHC cell blocks

Our small private facility which does app.. 10,000 cases per year is
experiencing difficulty with an increase in background on various antibodies
with cell block preparations.

Our cytology department rinses the button in cytolyte then adds 2-3 drops of
thrombin and plasma per their protocol.  We realize the proteins are a
problem.  We would like to know if there is another protocol for the
preparation of cell blocks minus the plasma and thrombin. 

Help please!


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