Processing cell monolayer/ multi-layer cultures


Can anyone offer any advice on processing cell cultures, grown in monolayer 
or multi-layers with inserts / membranes?  Are there any special 
requirements?  Would a general processing schedule used for animal (mainly 
murine & rat) tissues be OK?  Or would this be too rigorous, since the cell 
layers are more delicate in nature than lumps of tissue?  We use an 
overnight schedule of 16 hours without vacuum.   Is there such a thing as 
over-processing?  I read in the archives that overprocessing is a myth. 
 What are the symptoms that people claim are due to overprocessing? Any 
help or advice would be much appreciated.

Thank you

Nicola Cragg
Epistem Ltd,
Manchester, UK 

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