Processing cell monolayer/ multi-layer cultures
Can anyone offer any advice on processing cell cultures, grown in monolayer
or multi-layers with inserts / membranes? Are there any special
requirements? Would a general processing schedule used for animal (mainly
murine & rat) tissues be OK? Or would this be too rigorous, since the cell
layers are more delicate in nature than lumps of tissue? We use an
overnight schedule of 16 hours without vacuum. Is there such a thing as
over-processing? I read in the archives that overprocessing is a myth.
What are the symptoms that people claim are due to overprocessing? Any
help or advice would be much appreciated.
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