Liquid nitrogen problem

Dear all,

I am new to this work and if somebody could help me with this 
problem I would be very grateful. 
We are going to obtain skeletal muscle samples from surgery 
which we want to use for immunohistochemistry and in situ 
hybridization. We wanted to mount the muscle sample on cork in 
OCT and freeze it in isopentane/ liquid nitrogen there at the 
hospital. However there is no facility for getting liquid nitrogen at 
the hospital and transporting it there would be difficult. My question 
is would it be possible to freeze the muscle sample on dry ice, 
transport it back to the lab and then freeze in isopentane/liquid 
nitrogen? Could this sample still be used for immunohistochemistry 
and in situ hybridization?

Thanks to anyone that can help!

Maria Doyle
PhD Candidate
University College Dublin,

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