Infarct and Trichrome Questions
I am new at performing histology slides and have been appointed to do this
for my lab. My current project is looking at the apoptotic rate of murine
heart sections. I need a way to test and locate the area of the infarct
at a cellular level. The following protocol that is used on the slides is
a TUNEL assay kit. A friend suggested that I try using Massons TriChrome
Blue. This would stain the infarct area blue from the collagen. But I am
cant figure out how long it would take before the collagen begins to form
following the infarct. I am looking at the hearts roughly 12-16 hours after
the infarct was induced. Any thoughts as to how I can locate the infarct????
Help would be greatly apreciated
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