ICC on Cytospins

From:LINDA MARGRAF MD

Here's a message Ronnie is having trouble posting to the list. 
Please reply to the list or to him directly. Thanks.

From: Houston, Ronnie 
Sent: Monday, March 31, 2003 9:45 AM
To: Histonet (E-mail)
Subject: ICC on Cytospins


Could you share what fixation parameters you are using on cytospins for
ICC;
there are conflicting reports in the literature about what is best for
different antibodies, and it is impractical to have different fixation
protocols for each antibody, so what is being commonly employed?
At present, our cytospins are received in 95% alcohol; and this brings
up
the related question of adequate control material being identically
processed?

Thanks
Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com



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