H&E problem

From:Michele Margiotta

To All:

Just wondering if anyone can shed some light on a problem we've been having with our H&E stain. We are using a leica linear stainer with Harris hematoxylin.  The pathologists have noticed that the nuclei look vacuolated and have a hazy look to them.  We sent the blocks to another lab to stain and they look fine so, we know its not a problem with processing. Anyone have any suggestions?



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