[Fwd: Re: Renal tissue processing and sectioning]
>
> From: Angela M. Schmidt
> Date: 2003/03/21 Fri PM 09:44:15 EST
> To: "Muscato, Marilou"
> Subject: Re: Renal tissue processing and sectioning
>
>
> Marilou,
> We use the following procedure for processing our renal biopsies. I have heard no complaints from the pathologists and the sections seem to cut well. I have better luck sectioning on a Leitz microtome with Surgipath 123 blades than I do anything else. Good Luck!!
> Angie Schmidt
>
> Mossman's ----------15 min Kept warm in the cassette portion of the embedding center
> 70% alcohol--------- 6 min
> 95% alcohol--------- 6 min
> 100 % alcohol----- 6 min
> Acetone--------------6 min
> xylene---------------10 min
> paraffin-------------15 min( we use Paraplast Xtra)
> paraffin-------------15 min
>
>
>
>
> Angela M. Schmidt HT(ASCP)
> Lead Histology Technician
> TriHealth Laboratories
> Good Samaritan-Bethesda Hospitals
> Cincinnati, Ohio
> 513-872-1508
> a.schmidt@fuse.net
>
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