[Fwd: Re: Renal tissue processing and sectioning]

From:"Angela M. Schmidt"


> 
> From: Angela M. Schmidt 
> Date: 2003/03/21 Fri PM 09:44:15 EST
> To: "Muscato, Marilou" 
> Subject: Re: Renal tissue processing and sectioning
> 
> 
> Marilou, 
>   We use the following procedure for processing our renal biopsies. I have heard no complaints from the pathologists and the sections seem to cut well. I have better luck sectioning on a Leitz microtome with Surgipath 123 blades than I do anything else. Good Luck!!
>                    Angie Schmidt
> 
> Mossman's ----------15 min Kept warm in the cassette portion of the embedding center
> 70% alcohol--------- 6 min
> 95% alcohol--------- 6 min
> 100 % alcohol-----   6 min
> Acetone--------------6 min
> xylene---------------10 min
> paraffin-------------15 min( we use Paraplast Xtra)
> paraffin-------------15 min
> 
> 
> 
> 
> Angela M. Schmidt HT(ASCP)
> Lead Histology Technician
> TriHealth Laboratories
> Good Samaritan-Bethesda Hospitals
> Cincinnati, Ohio
> 513-872-1508
> a.schmidt@fuse.net
> 




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