Fwd: (Re: Mounting medium jars)

From:Ian Montgomery

Jill,
        Been using the same system as Louise for many years now. Rather than an accident with a syringe I was finding that glass pipettes would break at the tip leaving small shards of glass in the mounting media. These would eventually find their way onto the slide causing all sorts of problems.
Ian.


Date: Thu, 27 Mar 2003 10:25:27 +0000
From: louise renton <louise_renton@hotmail.com>
Subject: (Re: Mounting medium jars)
To: histonet@pathology.swmed.edu

Dear Jill

We have for a long time, (since an unfortunate accident with a glass syringe) used 3ml plastic Pasteur pipettes as "droppers" for mounting media.
They have the advantage of being relatively inexpensive,  require no cleaning (dispose of once the mounting is done) and that the droplet size can be adjusted by judicious trimming of the end.  They can - clean ones I mean - also double up as little chemical scoops if the bulb end is cut off horizontally

I have no idea, though, whether this syetm would be acceptable by cautious USA standards.

Louise
BRU, MRC
Johannesburg
South Africa

From: "J. A. Kiernan" <jkiernan@uwo.ca>
To: jill cox <jcox90@yahoo.com>
CC: histonet@pathology.swmed.edu
Subject: Unduly long (Re: Mounting medium jars)
Date: Thu, 27 Mar 2003 01:35:12 -0500

I've never owned a balsam bottle of my own, but have used
them, and never liked them.  It was easier and less
messy to make blunt-ended glass rods and dip them
into the bottle of mountant. When not in use, the
glass rod rested in the last (pre-coverslip) tank of
xylene. This was good because (a) the end of the rod
was always clean - didn't get bigger with accumulated
goo, and (b) the small amount of mountant dissolved in
the last tank of xylene increased its sensitivity to
carried-over alcohol, indicated by opalescence. Then
and now, you don't mount from cloudy xylene.

Since abandoning glass rods (see below), I've probably
seen more slides with obvious contamination of the mountant
with alcohol. There always were plenty of these because
students didn't look critically at their slides until they
needed high quality photos to put in their theses.
Nevertheless, I have a feeling in my urine that the
future of manual coverslipping is not with either the
lab-forged glass rod or the commercial balsam bottle,
but with the squeeze-controlled medium dispenser.

My advice is therefore:
If you can't find just a few balsam bottles,
consider buying a mountant that comes in a
plastic bottle that you invert and squeeze.

I discovered these a few years ago, and since
then I've hardly touched a glass rod. The medium
I use is called Cytoseal; it's in the Fisher
Scientific catalogue, and the label indicates
that the main ingredient is poly(methylmethacrylate).
It doesn't behave quite as nicely as Entellan, a
much more expensive poly(methylmethacrylate)
mountant that needs glass rodding, but it's OK.

Unless you make your own DPX it's necessary to live
with some trade secrecy with synthetic resinous
mounting media. Probably there are mountants other
than Cytoseal that come pre-packaged in ready-to-use
modular combined storage and application units.

Even 10-thumbed new graduate students (who call it
glue) quickly learn to apply the right amount of
mountant from a squeezy bottle without getting it
all over the place and sticking slides permanently
onto benches and hotplates. (The name glue came from
this unwanted action of mounting medium that dripped
unseen from the ends of the glass rods.)

This doesn't answer your question, but it might help.
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/
_____________________________________________________
jill cox wrote:
>
>   Hello everyone,
>       I have been looking for the mounting medium jars
> for coverslipping and cant find anything except one
> brand where you have to order a case at a time. They
> are too expensive that way. Does anyone know where I
> can get 2 to 3 of them? Thanks in advance
>
> =====
> Jill Cox HT (ASCP)
> Seattle Histology Lab

Is there only one histology lab in Seattle?
_____________________________________________________

_________________________________________________________________


Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk


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