Bcl-2 staining in decalcified rat tissue

From:case sinclair

Hello all.
I have just started a new project which involves staing some decalcified rat 
bone for Bcl-2 and Bax. The Bax is staining very well, but I cannot get 
any Bcl-2 staining, even in our controls (rat gut). We are using the Santa Cruz 
BCl-2 (c-2):sc7382 which is mouse anti-human, but the product info states that 
is reacts with rat.
Does anyone have any ideas or tips ?


Case Sinclair
Dept. of Orthopaedic Surgery, 
& Bone Growth Foundation
Women's and Children's Hospital Adelaide
72 King William Road
North Adelaide, SA 5006

Phones: (618) 8161 8042
Fax:      (618) 8161 7057
Email:    case.sinclair@adelaide.edu.au 

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