storage frozen tissue -80C

From:Linda Jenkins (by way of Histonet)

         There is hope if you can't do it NOW!  We routinely store fresh
tissue for very long time periods.  Place the tissue in a 30% sucrose
solution for 24 hours with vacuum and stirring.  Then embed the tissue in a
peel-a-way plastic mold in which there is a thin, pre-frozen layer of
freezing media, cover with more freezing medium, allow to freeze in a
cryostat, etc. (~ minus 30 C.), wrap in aluminum foil, store in air tight
plastic bag and place in - 80 freezer and label in LARGE letters with
pertinent info.  When you finally decide what stain to use and want to
remove the tissue from the - 80 freezer, remember to let the frozen
specimen acclimate to the cryostat temp for several hours before
sectioning.  If you don't... you get very brittle shards of tissue...too cold!
         Word of warning**  This does not cover the "rotter's" who throw
your tissue out or turn off the power.  We do have our - 80 freezers on
critical power.  Unfortunately, we still have people who get the urge to
clean without notifying anyone:-(
         Best Wishes,

Linda Jenkins, HT
Clemson University
Dept. of Bioengineering
Clemson, SC 29634-0905

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