f-actin labelling

From:tom.read@utoronto.ca

Hello,

I have been trying to label the f-actin, using phalloidin-rhodamine, of human 
eye tissue (endothelia of Schlemm's Canal) that has been stored in 10% buffered 
formalin for 5-6 years, with absolutely no success. I've always had excellent 
results using the same fluorephore to label tissue which has been stored for 1-2 
years in 1% formalin and am surprised at the poor results with this older 
tissue. Any comments or suggetions would be appreciated. Thanks.

Tom


-- 
Thomas Read, PhD
Glaucoma Research Lab
Gage Institute of Research
University of Toronto
223 College St., Rm 308
Toronto, ON  M5T 1R4  Canada

E-mail: tom.read@utoronto.ca







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