f-actin labelling



I have been trying to label the f-actin, using phalloidin-rhodamine, of human 
eye tissue (endothelia of Schlemm's Canal) that has been stored in 10% buffered 
formalin for 5-6 years, with absolutely no success. I've always had excellent 
results using the same fluorephore to label tissue which has been stored for 1-2 
years in 1% formalin and am surprised at the poor results with this older 
tissue. Any comments or suggetions would be appreciated. Thanks.


Thomas Read, PhD
Glaucoma Research Lab
Gage Institute of Research
University of Toronto
223 College St., Rm 308
Toronto, ON  M5T 1R4  Canada

E-mail: tom.read@utoronto.ca

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