Fellow netters,
I am attempting beta gal immunos on mouse tissue and getting a lot of background stain. I have tried 3% H202 as is and made up from 30% H2O2 in methanol. I have also tried different antibody titration's from 1:200 up to 1:4000. I am redoing the 1:2000 and 1:3000 tomorrow but need some suggestions on how to reduce the background staining. I also use the recommended 3% goat serum.
Thanks,
Ron Martin