having some trouble with our Bovine Viral Diarrhea (BVD) IHC
staining. We get great results staining gut tissues, but the ear notches
have stopped working. We use known positive and known
negative tissues for our controls. The weird thing is, both were
working just peachy keen at 1:2000, then the ear notch tissues stopped working,
but the gut tissues have continued to stain just fine. We have had to
increase the ab concentration to 1:500 just last week. Our antibody is from
Oklahoma State U., mouse ascites fluid.
there anyone who my have some clue why the antibody will work on one tissue and
not another even though it used to work fine on both?