Re: staining for mucin on frozen sections
Andrea Grantham wrote:
> Good morning! Went out of town to a wedding this weekend and still feeling
> a bit fuzzy this morning - the question has come up about doing stains for
> mucin on frozen sections. If you cut the frozens and fix in 10% formalin
> would you get the same results as with paraffin?
Yes, but with some staining methods you can expect some
glycolipids to be stained as well as the mucus. For example,
myelin is quite strongly PAS-positive in frozen sections
but only weakly so after dehydration and paraffin embedding.
You could avoid this by fixing the cryosections in alcohol
instead of formalin.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
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