I haven't used CD3 on mouse lung, but I have found that the ARK kit from DAKO works wonders with mouse monoclonals and mouse tissue.
It's extremely efficient, and I wish I could use it for all my other antibodies, i.e., rat, etc. The stain takes about 1 hour start to finish, and is sharp and crisp.
No need to use only DAKO antibodies, either.
Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
847.938.4919
Fax 847.938.3266
Russ Ellis <techmanruss@yahoo.ca>
03/04/2003 12:53 PM
To: histonet@pathology.swmed.edu
cc:
Subject: mouse lung CD3 immunostain
Hello everyone
I am developing a method for staining formalin-fixed,
paraffin-embedded mouse lungs with an antibody to CD3.
After an initial review of the archives I was hoping
for some feedback with the following:
1. ANTIBODY - Dako, Serotec, Novocastra, other(?).
I'm sure they all work to some degree, but what are
some of the negative aspects?
2. CITRATE BUFFER - should I be concerned about the
length of microwaving time given that lungs are more
fragile than most other tissues?
3.DAKO ARK - does anyone have any experience with this
"time saving" kit that they could provide some pros
and cons? For me, time isn't a factor if the results
are poor.
I look forward to your useful insights. If anyone
knows of a method that they can point me to as a place
to start, I would be very grateful.
Regards,
Russ Ellis
=====
Russ Ellis, B.Sc., M.L.T.
Research Lab Coordinator
St. Joseph's Hospital
Hamilton, Canada
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