I haven't used CD3 on mouse lung, but I have found that the ARK kit from DAKO works wonders with mouse monoclonals and mouse tissue.
It's extremely efficient, and I wish I could use it for all my other antibodies, i.e., rat, etc. The stain takes about 1 hour start to finish, and is sharp and crisp.
No need to use only DAKO antibodies, either.
Jacqueline M. O'Connor HT(ASCP)
Global Pharmaceutical Research and Development
Russ Ellis <email@example.com>
03/04/2003 12:53 PM
Subject: mouse lung CD3 immunostain
I am developing a method for staining formalin-fixed,
paraffin-embedded mouse lungs with an antibody to CD3.
After an initial review of the archives I was hoping
for some feedback with the following:
1. ANTIBODY - Dako, Serotec, Novocastra, other(?).
I'm sure they all work to some degree, but what are
some of the negative aspects?
2. CITRATE BUFFER - should I be concerned about the
length of microwaving time given that lungs are more
fragile than most other tissues?
3.DAKO ARK - does anyone have any experience with this
"time saving" kit that they could provide some pros
and cons? For me, time isn't a factor if the results
I look forward to your useful insights. If anyone
knows of a method that they can point me to as a place
to start, I would be very grateful.
Russ Ellis, B.Sc., M.L.T.
Research Lab Coordinator
St. Joseph's Hospital
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