Lilia,
Could it be an antibody problem?  I've had a lot of success using the
CM5 anti-p53 antibody from Novocastra on ffpe mouse intestine.  I guess
I don't know how it would work on frozen sections, though.
Matt

Matt McElwee
Research Specialist
Department of Surgery
K4/617 Clinical Science Center
600 Highland Avenue
Madison, WI 53792
(608) 263-7648
mcelwee@surgery.wisc.edu

>>> Lilia Muolo  (by way of Histonet)  03/07/03
10:25PM >>>
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    Hi, I am having trouble staining frozen section mouse tissue  with
P53
antibodies. My human colon cancer tissue stains just fine but no dice 
with
the mice. Please advise if you have any good tips. Thanks, Lil Cancer
Institute of New Jersey