Novocastra offers NCL-PCNA.  You can see the data sheet on their website:
www.novocastra.co.uk

----- Original Message -----
From: "Demirs, John T." 
To: "Histo Net (E-mail)" 
Sent: Tuesday, March 04, 2003 7:25 AM
Subject: RE: RE: Immunostain for PCNA


> Dako is your best bet. If they don't have what you want then I go to
secondary
> sources,no order of preference here, B D Pharmingen, Biogenex, BD
biosciences,
> R&D sciences, Novo castra.
> Try this web site, click on "Antibody suppliers" , you'll have over 200 to
> choose from, http://www.antibodyresource.com/
>
> John T. Demirs B. A. HTL (ASCP)
> Wellman Laboratories of Photomedicine
> Massachusetts General Hospital
> Photopathology Dept. Edward 214
> 15 Fruit St.
> Boston Ma 02114
> 617-726-6984 phone
> 617-726-1206 fax
>
> > -----Original Message-----
> > From: NIDAL E MUVARAK [SMTP:nmuvarak@facstaff.wisc.edu]
> > Sent: Sunday, March 02, 2003 8:04 PM
> > To: Demirs, John T.
> > Subject: Re: RE: Immunostain for PCNA
> >
> > Thanks for confiming my thoughts. I have repeatedly tried to tell my PI
that
> > Santa Cruz primaries are not the best, but she doesn't want to switch to
> > anything else since her old tech spent a month trying to figure out the
right
> > dilution ( I looked at some of his slides and none of them impressed
me). Just
> > out of curiousity, where do you get your primary for PCNA from? Thanks.
> >
> > Nidal E Muvarak
> > Associate Research Specialist
> > Department of Biomedical Engineering
> > University of Wisconsin-Madison
> > 1550 Engineering Dr.; Rm. 2158
> > Madison, WI 53706-1609
> > Lab: (608) 265-8921; Office: (608) 265-4205;
> > Home: (608) 256-7934; Cell: (608) 332-6068
> >
> > ----- Original Message -----
> > From: "Demirs, John T." 
> > Date: Friday, February 28, 2003 1:28 pm
> > Subject: RE: Immunostain for PCNA
> >
> > > My first reactions is not to use Ab from Santa Cruze. I have never
> > > found any of
> > > their primaries to be any good.
> > > Having said that, We use overnight incubations at 4C, tittered out to
> > > 1:200-1:500. You should be doing Antigen Retrieval for nuclear
> > > staining and for
> > > controls any tissue should have some level of cell proliferation
> > > some are better
> > > then others i.e. liver, spleen, skin.
> > > John T. Demirs B. A. HTL (ASCP)
> > > Wellman Laboratories of Photomedicine
> > > Massachusetts General Hospital
> > > Photopathology Dept. Edward 214
> > > 15 Fruit St.
> > > Boston Ma 02114
> > > 617-726-6984 phone
> > > 617-726-1206 fax
> > >
> > > > -----Original Message-----
> > > > From: NIDAL E MUVARAK [SMTP:nmuvarak@facstaff.wisc.edu]
> > > > Sent: Thursday, February 27, 2003 3:14 PM
> > > > To: HistoNet Server
> > > > Subject: Immunostain for PCNA
> > > >
> > > > Hi. I was wondering if anyone has any suggestions on a protocol
> > > for PCNA
> > > > stain. I'm staining mouse lung tissue using the Envision+ kit
> > > from DAKO. I'm
> > > > using a rabbit anti-PCNA from Santa Cruz biotech as the primary
> > > Ab. I incubate
> > > > at RT for 1hr. Everything else is done as suggested by the kit
> > > (peroxidase> block, serum block, secondary Ab, chromogen, etc). I
> > > used the PCNA Ab at 1:50
> > > > and got nothing. When I went to 1:25, I had a lot of backrgound
> > > and no stain
> > > > in the nucleus whatsoever. I'm wondering if anyone has any
> > > suggestions on this
> > > > stain, and also what is the best tissue from mouse to use as a
> > > positive> control. Thank you for your time.
> > > >
> > > > Nidal E Muvarak
> > > > Associate Research Specialist
> > > > Department of Biomedical Engineering
> > > > University of Wisconsin-Madison
> > > > 1550 Engineering Dr.; Rm. 2158
> > > > Madison, WI 53706-1609
> > > > Lab: (608) 265-8921; Office: (608) 265-4205;
> > > > Home: (608) 256-7934; Cell: (608) 332-6068
> > > >
> > > >
> > > >
> > >
> > >
>
>