Re: Quality of Fixation
|From:||Linda Jenkins (by way of Histonet)|
"Is there a standard measure of quality of fixation? A stain that shows
degree of fixation? I don't have a lot of equipment, and don't want to
spend a lot of money, but I have been challenged by a reviewer to show
that my perfusion methods (animal research) results in as good a
fixation as standard methods.
I can see it does, tissue quality is excellent for sectioning and
staining, but how do I quantify or show that? Age it 90 days and
report degree of crumbling? It is 4% formaldehyde fixation."
There are tons of really good references spouting the efficacy of perfusion
fixation over "standard methods". Seems I recall several articles in the
Journal of Histotechnology on this same topic. I am going to send you a
picture that I took of perfused versus non-perfused femurs. This picture
really shows the difference! I think we also have a way to load on a
histonet picture site? If some kind soul would share that info, I will
also place it on that site.
After reviewing your question I wonder...is the reviewer questioning your
"choice of perfusion method" or "perfusion versus immersion" (there is NO
comparison between these two methods)?
Linda Jenkins, HT
Dept. of Bioengineering
Clemson, SC 29634-0905
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